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Image Search Results


Journal: STAR Protocols

Article Title: CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models

doi: 10.1016/j.xpro.2021.101038

Figure Lengend Snippet:

Article Snippet: The plasmid map was generated using SnapGene software (from Insightful Science; available at snapgene.com ). e. Store the plasmid at −20°C (> 5 years) or short-term at +4°C. f. Once you have verified your pX330-sgRNA plasmid, you can proceed to perform a midipreparation of your plasmid using the Thermo Fisher Scientific PureLink TM HiPure Plasmid Midiprep kit according to manufacturer’s instructions ( https://assets.fishersci.com/TFS-Assets/LSG/manuals/purelink_hipure_plasmid_dna_purification_man.pdf ). g. After midipreparation of your plasmid DNA, measure the concentration using a NanoDrop spectrophotometer. h. The target selector plasmid generated in this step is named pX330-Cdk4_Ct.

Techniques: Purification, Recombinant, Binding Assay, Lysis, Staining, Transfection, DNA Purification, Plasmid Preparation, DNA Extraction, Sequencing, Software, Spectrophotometry